Their initial hypothesis was that Ca might offer defensive benefits against Cu-induced toxicity in the selected fish species, known as Pelteobagrus fulvidraco, specifically by altering certain enzymatic functions. To test this hypothesis, the extensive research team exposed yellowish catfish to 0, 1, and 2 milligrams of Cu per liter , in conjunction with 0 and 50 mg of Ca/l. As predicted, exposure to these chemicals at these varying degrees of both Cu and Ca demonstrably affected specific enzymatic activities in the seafood, including succinate dehydrogenase, malic dehydrogenase, lactate dehydrogenase, lipoprotein lipase and hepatic lipase.Though it could hereditary be, but factors such as for example sitting for long intervals on uneven or hard chairs, straining during defecation, and suppressing or early discharge of gas, urine or faeces cause it. Sleeping at irregular hours and leading a sedentary life-style also breed of dog the symptoms of piles. Therefore, to avoid piles you need to: * Soften your stools to allow them pass easier and the simplest way to get this done is to add water and fiber to your daily diet and minimize your intake of processed foods.